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Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( <t>https://www.ncbi.nlm.nih.gov/nuccore/KX011115</t> ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end <t>nucleotide</t> positions within the KX01115 sequence.
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Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( <t>https://www.ncbi.nlm.nih.gov/nuccore/KX011115</t> ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end <t>nucleotide</t> positions within the KX01115 sequence.
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Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( <t>https://www.ncbi.nlm.nih.gov/nuccore/KX011115</t> ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end <t>nucleotide</t> positions within the KX01115 sequence.
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Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( <t>https://www.ncbi.nlm.nih.gov/nuccore/KX011115</t> ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end <t>nucleotide</t> positions within the KX01115 sequence.
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Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( https://www.ncbi.nlm.nih.gov/nuccore/KX011115 ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end nucleotide positions within the KX01115 sequence.

Journal: bioRxiv

Article Title: On the sensitivity of qPCR diagnostics for the canola clubroot pathogen Plasmodiophora brassicae

doi: 10.64898/2026.02.05.704046

Figure Lengend Snippet: Diagrammatic sketch showing the genomic locations of targets of PCR and qPCR systems used for detection of the clubroot pathogen Plasmodiophora brassicae . KX01115 is the GenBank accession number for a partial ribosomal DNA (rDNA) sequence from a P. brassicae strain ( https://www.ncbi.nlm.nih.gov/nuccore/KX011115 ). gBlock is a synthesized double-stranded DNA fragment used to evaluate the sensitivity of three probe-based qPCR systems. Numbers flanking each region indicate the start and end nucleotide positions within the KX01115 sequence.

Article Snippet: The target regions of the primers were obtained by BLAST searching the primer sequences against the National Center for Biotechnology Information (NCBI) nucleotide collection (nr/nt) database ( https://blast.ncbi.nlm.nih.gov/Blast.cgi ).

Techniques: Sequencing, Synthesized